Compound microscopes are primarily used today to focus on sequential focal planes. The translation of 2D and 3D has always been a roadblock in biology. As we all know, life is three dimensional and so biology should be studied in a three dimensional realm.
Light microscopy, otherwise known as fluorescent microscopy has provided insight into a three dimensional cellular structure through light optics. The major downfall to this procedure is that only specimens must be composed of the thinnest samples in order to be visible in a good image.Fluorescence has since fixed the flaws of traditional light microscopy by offering higher resolution and light from axial focal plans.
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